Antibiofilm Activity of Methanol Extract of Rumex dentatus Against Pseudomonas aeruginosa

Authors

  • Maryam Pezeshki Najafabadi Mahdieh Medical Diagnosis Charity Center, Iran
  • Maryam Mohammadi-Sichani Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
  • Mohammad Javad Kazemi Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran
  • Mohammad Sadegh Shirsalimian 5Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran
  • Majid Tavakoli Lorestan Agricultural and Natural Resources Research Center, Khorramabad, Lorestan, Iran

DOI:

https://doi.org/10.37819/biosis.001.01.0044

Keywords:

Pseudomonas aeruginosa, Plant extracts, Urinary Tract Infections, Burns, Biofilm

Abstract

Biofilm formation of Pseudomonas aeruginosa makes up a sizeable proportion of hospital-acquired infections, because bacteria in biofilms can resist antibiotic treatment. The extracellular polymeric substance of P. aeruginosa biofilm is an imprecise collection of extracellular polysaccharides, proteins and microbial cells. Rumex dentatus belongs to polygonaceae family. This family can be found in Middle East. The aim of this present study was to assess the effect of various concentrations of methanol extract of Rumex dentatus on biofilm formation of Pseudomonas aeruginosa after 48 h and 72 h. In this experimental study we collected Rumex dentatus from Khoramabad, Iran. The working extracts were 250, 125, 62.5, 31.25, 15.62, 7.81, 3.9, 1.95, 0.97 and 0.48 mg/ml. We used microtiter plate method to grow P. aeruginosa biofilm and assess the antibiofilm activity of plant extract. The composition of methanol extract obtained from Rumex dentatus was studied by gas chromatography. The minimum biofilm inhibitory concentration (MBIC) for P. aeruginosa found to be 250 mg/ml. GC-MS  analyses indicated that these fractions contained a variety of compounds including Bicyclo (3.1.1) heptan- 3 -one, 2, 6, 6- trimethyl,  Bicyclo (3.1.1) heptan, 6, 6- dimethyl and Eucalyptol. There were consequential correlations between antibiofilm activity and the concentration of extracts after 48 and 72 h.

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References

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Biosis: Biological Systems

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Published

2020-03-04

How to Cite

Pezeshki Najafabadi, M., Mohammadi-Sichani, M. ., Kazemi, M. J., Shirsalimian, M. S. ., & Tavakoli, M. . (2020). Antibiofilm Activity of Methanol Extract of Rumex dentatus Against Pseudomonas aeruginosa. Biosis: Biological Systems, 1(1), 25–32. https://doi.org/10.37819/biosis.001.01.0044

Issue

Vol. 1 No. 1 (2020): Biosis: Biological Systems

Section

Original research

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Authors retain copyright over the materials, which are published under an Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) license.