Skip to main content Skip to main navigation menu Skip to site footer
  • \
    Start Submission Become a Reviewer

    Antibiofilm Activity of Methanol Extract of Rumex dentatus Against Pseudomonas aeruginosa



    Abstract

    Biofilm formation of Pseudomonas aeruginosa makes up a sizeable proportion of hospital-acquired infections, because bacteria in biofilms can resist antibiotic treatment. The extracellular polymeric substance of P. aeruginosa biofilm is an imprecise collection of extracellular polysaccharides, proteins and microbial cells. Rumex dentatus belongs to polygonaceae family. This family can be found in Middle East. The aim of this present study was to assess the effect of various concentrations of methanol extract of Rumex dentatus on biofilm formation of Pseudomonas aeruginosa after 48 h and 72 h. In this experimental study we collected Rumex dentatus from Khoramabad, Iran. The working extracts were 250, 125, 62.5, 31.25, 15.62, 7.81, 3.9, 1.95, 0.97 and 0.48 mg/ml. We used microtiter plate method to grow P. aeruginosa biofilm and assess the antibiofilm activity of plant extract. The composition of methanol extract obtained from Rumex dentatus was studied by gas chromatography. The minimum biofilm inhibitory concentration (MBIC) for P. aeruginosa found to be 250 mg/ml. GC-MS  analyses indicated that these fractions contained a variety of compounds including Bicyclo (3.1.1) heptan- 3 -one, 2, 6, 6- trimethyl,  Bicyclo (3.1.1) heptan, 6, 6- dimethyl and Eucalyptol. There were consequential correlations between antibiofilm activity and the concentration of extracts after 48 and 72 h.

    Keywords: Pseudomonas aeruginosa Plant extracts Urinary Tract Infections Burns Biofilm
    Section

    References

    1. Abou Elfotoh M.A., K.A. Shams, K.P. Anthony, A.A. Shahat, M.T. Ibrahim, N.M. Abdelhady, N.S. Abdel Azim, F.M. Hammouda, M.M. El-Missiry, & M.A. Saleh. (2013). Lipophilic constituents of Rumex vesicarius L. and Rumex dentatus L. Antioxidants, 2, 167-180. https://doi.org/10.3390/antiox2030167
    2. Alikhani M.Y., Z. Karimi Tabar, F. Mihani, E. Kalantar, P. Karami, M. Sadeghi, S. Ahdi Khosroshahi, & S. Farajnia. (2014). Antimicrobial resistance patterns and prevalence of blaPER-1 and blaVEB-1 Genes Among ESBL-producing Pseudomonas aeruginosa isolates in west of Iran. Jundishapur Journal of Microbiology, 7, e8888. https://dx.doi.org/10.5812%2Fjjm.8888
    3. Azizi O., M.R. Shakibaie, F. Modarresi, & F. Shahcheraghi. (2015). Molecular detection of Class-D OXA carbapenemase genes in biofilm and non-biofilm forming clinical isolates of Acinetobacter baumannii. Jundishapur Journal of Microbiology, 8, e21042. https://dx.doi.org/10.5812%2Fjjm.21042
    4. Bacalso M., T. Xu, K. Yeung, & D. Zheng. (2011). Biofilm formation of Pseudomonas aeruginosa PA14 required lasI and stimulated by the Pseudomonas quinolone signal although salicylic acid inhibition is independent of the pqs Pathway. Journal of Experimental Microbiology and Immunology, 15, 84-89.
    5. Cardoso N., T.T. Cavalcante, A.X. Araujo, H.S. Santos, M.R. Albuquerque, P.N. Bandeira, R.M. Cunha, B.S. Cavada, & E.H. Teixeira. (2012). Antimicrobial and antibiofilm action of casbane diterpene from Croton nepetaefolius against oral bacteria. Archives of Oral Biology, 57, 550-555. https://doi.org/10.1016/j.archoralbio.2011.10.016
    6. Elzaawely A.S. & Tawata. (2012). Antioxidant capacity and phenolic content of Rumex dentatus L. grown in Egypt. Journal of Crop Science and Biotechnology, 15, 59-64. https://doi.org/10.1007/s12892-011-0063-x
    7. Fatima N., M. Zia, R. Rehman, Z. Rizvi, S. Ahmad, B. Mirza, & M. Chaudhary. (2009). Biological activities of Rumex dentatus L: evaluation of methanol and hexane extracts. African Journal of Biotechnology, 8, 6945-6951.
    8. Hameed I.G. Dastagir. (2009). Nutritional analyses of Rumex hastatus D. Don, Rumex dentatus Linn and Rumex nepalensis Spreng. African Journal of Biotechnology, 8, 4131-4133.
    9. Harmsen M., L. Yang, S.J. Pamp, & T. Tolker-Nielsen. (2010). An update on Pseudomonas aeruginosa biofilm formation, tolerance, and dispersal. FEMS Immunology & Medical Microbiology, 59, 253-268. https://doi.org/10.1111/j.1574-695X.2010.00690.x
    10. Hussain F., B. Ahmad, I. Hameed, G. Dastagir, P. Sanaullah, & S. Azam. (2010). Antibacterial, antifungal and insecticidal activities of some selected medicinal plants of polygonaceae. African Journal of Biotechnology, 9, 5032-5036.
    11. Kim H.-S., & H.-D. Park. (2013). Ginger extract inhibits biofilm formation by Pseudomonas aeruginosa PA14. PLoS ONE, 8, e76106. https://dx.doi.org/10.1371%2Fjournal.pone.0076106
    12. Lihua L., W. Jianhuit, Y. Jialini, L. Yayin, & L. Guanxin. (2013). Effects of allicin on the formation of Pseudomonas aeruginosa biofilm and the production of quorum-sensing controlled virulence factors. Polish Journal Microbiology. 62, 243-251.
    13. Mittal R., S. Aggarwal, S. Sharma, S. Chhibber, & K. Harjai. (2009). Urinary tract infections caused by Pseudomonas aeruginosa: A mini review. Journal of Infection and Public Health, 2, 101-111. https://doi.org/10.1016/j.jiph.2009.08.003
    14. Mohanty S., S. Mishra, P. Jena, B. Jacob, B. Sarkar, & A. Sonawane. (2011). An investigation on the antibacterial, cytotoxic, and antibiofilm efficacy of starch-stabilized silver nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine, 8, 916-924. https://doi.org/10.1016/j.nano.2011.11.007
    15. Nascimento G.G.F., J. Locatelli, P.C. Freitas, & G.L. Silva. (2000). Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal Microbiology, 31, 247-256. https://doi.org/10.1590/S1517-83822000000400003
    16. Nisa H., A.N. Kamili, S.A. Bandh, S.-u. Amin, B.A. Lone, & J.A. Parray. (2013). Phytochemical screening, antimicrobial and antioxidant efficacy of different extracts of Rumex dentatus L. - A locally used medicinal herb of Kashmir Himalaya. Asian Pacific Journal of Tropical Disease, 3, 434–440. https://dx.doi.org/10.1016%2FS2222-1808(13)60097-3
    17. Oskay M., D. Oskay, & F. Kalyoncu. (2009). Activity of some plant extracts against multi-drug resistant human pathogens. Iranian Journal of Pharmaceutical Research, 8, 293-300.
    18. Pezeshki Najafabadi, M. , Mohammadi-Sichani, M., Kazemi, M. J., Shirsalimian, M., & Tavakoli, M. (2016). Investigation of the chemical composition and different effects of a Rumex dentatus methanol extract against drug resistant Pseudomonas aeruginosa isolates. Iranian Red Crescent Medical Journal, 18(12), e27064. http://dx.doi.org/10.5812/ircmj.27064
    19. Pitts B., Hamilton, M.A., Zelver, N., & Stewart, P.S. (2003). A microtiter-plate screening method for biofilm disinfection and removal. Journal of Microbiological Methods, 54, 269-276. https://doi.org/10.1016/S0167-7012(03)00034-4
    20. Stepanovic S., D. Vukovic, I. Dakic, B. Savic, & M. Svabic-Vlahovic. (2000). A modified microtiter-plate test for quantification of staphylococcal biofilm formation. Journal of Microbiological Methods, 40, 175-179. https://doi.org/10.1016/S0167-7012(00)00122-6
    21. Sutter V., & V. Hurst. (1966). Sources of Pseudomonas aeruginosa infection in burns: study of wound and rectal cultures with phage typing. Annals of Surgery, 163, 597–602. https://dx.doi.org/10.1097%2F00000658-196604000-00013
    22. Taj M., H. Mukhtiar, S. Inayat Ur Rahman, F. Ijaz, F. Gul, A. Muhammad Afzal, S. Hassan, & Z. Ahmad. (2014). In-vitro antibacterial study of stem extract of Rumex dentatus against different bacterial pathogenic strains. American-Eurasian Journal of Agricultural & Environmental Sciences, 14, 199-202. https://dx.doi.org/10.5829/idosi.aejaes.2014.14.03.12309
    23. Varposhti M., A. Abdi Ali, P. Mohammadi, & A. Saboora. (2013). Effects of extracts and an essential oil from some medicinal plants against biofilm formation of Pseudomonas aeruginosa. Journal of Medical Microbiology and Infectious Diseases, 1, 36-40.
    24. Wadood H., & A. Sabri. (2013). Screening, characterization and biofilm formation of nickel resistant bacteria. Polish Journal of Microbiology, 62, 411-418.
    25. Walker T., H. Bais, E. Deziel, H. Schweizer, L. Rahme, R. Fall, & J. Vivanco. (2004). Pseudomonas aeruginosa-plant root interactions. Pathogenicity, biofilm formation, and root exudation. Plant Physiology, 134, 320-331. https://doi.org/10.1104/pp.103.027888
    26. Wiegand I., K. Hilpert, & R.E.W. Hancock. (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols, 3, 163-175. https://doi.org/10.1038/nprot.2007.521
    27. Yahya M., M. Irahim, W. Zawawi, & U. Hamid. (2014). Biofilm killing effects of Chromolaena odorata extracts against Pseudomonas aeruginosa. Research Journal of Phytochemistry, 8, 64-73. http://dx.doi.org/10.3923/rjphyto.2014.64.73

    How to Cite

    Pezeshki Najafabadi, M., Mohammadi-Sichani, M. ., Kazemi, M. J., Shirsalimian, M. S. ., & Tavakoli, M. . (2020). Antibiofilm Activity of Methanol Extract of Rumex dentatus Against Pseudomonas aeruginosa. Biosis: Biological Systems, 1(1), 25–32. https://doi.org/10.37819/biosis.001.01.0044
    ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver
    Download Citation
    Endnote/Zotero/Mendeley (RIS) BibTeX

    HTML
    422

    Total
    321

    Share

    Search Panel

    Maryam Pezeshki Najafabadi
    Google Scholar
    Pubmed
    JDMFS Journal

    Maryam Mohammadi-Sichani
    Google Scholar
    Pubmed
    JDMFS Journal

    Mohammad Javad Kazemi
    Google Scholar
    Pubmed
    JDMFS Journal

    Mohammad Sadegh Shirsalimian
    Google Scholar
    Pubmed
    JDMFS Journal

    Majid Tavakoli
    Google Scholar
    Pubmed
    JDMFS Journal

    Downloads

    Article Details

    DOI: https://doi.org/10.37819/biosis.001.01.0044

    Published: 2020-03-04

    Most Read This Month

    License

    Copyright © 2023 EurAsia Academic Publishing Group. All rights reserved