Omega-3 fatty acid protects against chemical induced liver injury- an experimental study
Abstract
INTRODUCTION: The mainstream detoxification of chemical agents is carried out in liver which is chief organ of body. Omega-3 is belonging to family of polyunsaturated fatty acids which has several beneficial effects on metabolism and inflammation.
OBJECTIVE: To assess to hepatoprotective effects of the Ω- 3-FA on carbon tetra chloride induced liver injury in laboratory animals at animal house.
METHODOLOGY: After ethical review committee approval this study was performed at the Department of Anatomy and Pathology, Bhitai Dental and Medical College and Animal house of Sindh Agriculture University Tando Jam. 80 Wistar albino rats were selected and randomly divided into 4 groups and drugs were given for allocated time. Blood sampling, biochemical and histopathological analysis were carried out.
RESULTS: The Ω- 3-FA and silymarin demonstrated protective effects against the CCl4 induced liver injury. Group C and D which were treated with the Ω- 3-FA and silymarin revealed significant amelioration of the liver function tests (p<0.05).
CONCLUSION: The rat models showed tremendous benefits of omega-3 on liver due to carbon tetrachloride induced damage.
KEY WORDS: Omega-3, Carbon tetrachloride, Hepatocellular injury
References
Khanchandani K, Singh SP, Agarwal A. Role of Omega-3 fatty acid in hepatoprotection against carbon tetrachloride induced liver injury in albino rats. J Biomed Pharmaceut Res 2014; 3 (6):131-5.
Meganathan M, Gopal MG, Sasikala P, Mohan J, Gowdhaman N, Balamurugan K, Nirmala P, et al. Evaluation of Hepatoprotective Effect of Omega 3- Fatty Acid against Paracetamol Induced Liver Injury in Albino Rats. Global J Pharmacol 2011; 5 (1): 50-53,
Essawy AE, Abdel-Moneim AM, Khayyat LI and Elzergy AA. Nigella sativa seeds protect against hepatotoxicity and dyslipidemia induced by carbon tetrachloride in mice. J Appl Pharm Sci 2012; 2 (10):021-5.
Wafay H, El-Saeed G, El-Toukhy S, Youness E, Ellaithy N, Agaibi M and Eldaly S. Potential effect of garlic oil and silymarin on carbon tetrachloride induced liver injury. Austr J Basic Appl Sci 2012; 6 (3):409-14.
Al-Razzuqi R. Hussaini J and Al-Jeboori A. Protective effect of Nigella sativa against carbon tetrachloride induced acute liver injury in experimental rabbit models. Int J Green Pharm 2011; 5(3): 198-200.
Alarifi S, Aldahmash B, El-Nagar D and Dkhil M. Effect of corn oil, flaxseed oil and black seed oil on lead acetate-induced hepatic tissue damage: A histological study. J Med Plants Res 2012; 6(24): 4128-34.
Feng R, Wang M, Yan C, Li P, Chen M, He C, Wan JB. Endogenous n-3 Fatty Acids Alleviate Carbon-Tetrachloride-Induced Acute Liver Injury in Fat-1 Transgenic Mice. Oxidative Med Cell Longevity 2016: Article ID 7962948: 1-12.
Sgroc Clinard, F. and K. Ouazrir. Incidence of drug induced hepatic injuries. A French population based study. Hepatol 2002; 36: 451-455.
Ismail AF, Salem AA, Eassawy MM. Hepatoprotective effect of grape seed oil against carbon tetrachloride induced oxidative stress in liver of ð›¾-irradiated rat. J Photochem Photobiol Biol 2016; 160: 1–10.
Murat-Bilgin H, Atmaca M, Deniz-Obay B, Ozekinci S, TaÅŸdemir E and Ketani A. Protective effects of coumarin and coumarin derivatives against carbon tetrachloride-induced acute hepatotoxicity in rats. Exp Toxicol Pathol 2011; 63(4): 325-30.
Bernhard Hennig, Gudrun Reiterer, Zuzana Majkova, Elizabeth Oesterling, Purushothaman Meerarani and Michal Toborek. Modification of environmental toxicity by nutrients Implications in atherosclerosis. Cardiovasc toxicol 2005; 5(2): 153-160,
Dong D, Xu L, Yin L, Qi Y, Peng J. Naringin prevents carbon tetrachloride-induced acute liver injury in mice. J Func Food 2015; 12:179–91.
Su C, Xia X, Shi Q. Neo hesperidin dihydrochalcone versus CCl4-induced hepatic injury through different mechanisms: the implication of free radical scavenging and Nrf2 Activation. J Agricult Food Chem 2015; 63 (22): 5468–75.
Kasote DM, Badhe YS, Zanwar AA, Hegde MV and Deshmukh KK, Hepatoprotective potential of ether insoluble phenolic components of n-butanol fraction (EPC-BF) of flaxseed against CCl4 -induced liver damage in rats. J Pharm Bioall Sci 2012; 4: 231 – 235.
Tang X, Gao J, Wang Y, Fan YM, Xu LZ and Zhao XN et al. Effective protection of Terminalia catappa L. leaves from damage induced by carbon tetrachloride in liver mitochondria. J Nutr Biochem 2006; 17: 177 – 182.
Undurti N Das Can essential fatty acids reduce the burden of disease(s)? Lipids Health Dis 2008; 7: 9.
Simopoulos AP, Omega-3 fatty acids in health and disease and in growth and development. Am J Clin Nutr 1991; 54: 438 – 463.
Naqshbandi A, Khan MW, Rizwan S, Yusufi ANK and Khan F. Studies on the protective effect of fish oil against cisplatin induced hepatotoxicity. Biol Med 2011; 3(2): 86 – 97.
Kalra J, Ali B, Kalra S and Pant KK. Fish oil and its role in acetaminophen induced hepatic injury. Asian J Exp Biol Sci 2012; 3(4): 826 – 829.
Ma JQ, Ding J, Zhang L, Liu M. Hepatoprotective properties of sesamin against CCl4 induced oxidative stress mediated apoptosis in mice via JNK pathway. Food Chem Toxicol 2014; 64: 41–48.
Kim K, Jung N, Lee K. Dietary omega-3 polyunsaturated fatty acids attenuate hepatic ischemia/reperfusion injury in rats by modulating toll-like receptor recruitment into lipid rafts. Clin Nutr 2-13; 32 (5): 855–62.
Yang BY, Zhang XY, Guan SW, Hua WC. Protective effect of procyanidin B2 against CCl4-induced acute liver injury in mice. Molecules 2015; 20 (7): 12250–65.
Racanelli V, Rehermann B. The liver as an immunological organ. Hepatology. 2006; 43: S54–S62.
Zhang XJ, Huang LL, Cai XJ, Li YT, Wang YT, Wan JB. Fatty acid variability in three medicinal herbs of Panax species. Chem Centr J 2013; 7 (1):1-12.
Luster MI, Simeonova PP, Gallucci RM, Bruccoleri A, Blazka ME, Yucesoy B. Role of inflammation in chemical-induced hepatotoxicity. Toxicol Lett. 2001; 120: 317–21.
Al-Attar AM, Al-Rethea HA. Chemoprotective effect of omega-3 fatty acids on thioacetamide induced hepatic fibrosis in male rats. Saudi J Biol Sci 2016; 1:1-10.
Al-Attar AM. Attenuating effect of Ginkgo biloba leaves extract on liver fibrosis induced by thioacetamide in mice. J Biomed Biotechnol 2012; 1:1–9.
Luo M, Dong L, Li J, Wang Y, Shang B. Protective effects of pentoxifylline on acute liver injury induced by thioacetamide in rats. Int`l J Clin Exp Path 2015; 8:8990–6.
Mansour DF, Nada SA, Eldenshary ES, Elmahmoudy BM, Abd-Elgayed SS. Antioxidant and hypo-ammonemic activities of alpha-lactalbumin and vitamin C in thioacetamide-induced liver and brain damage in rats. J Appl Pharm Sci 2015; 5:072–081.
Atakisi O, Atakisi E, Ozcan A, Karapehlivan M, Kart A. Protective effect of omega-3 fatty acids on diethylnitrosamine toxicity in rats. Eur Rev Med Pharmacol Sci 2013; 17:467–71.
Alaraj M, Qiblawi S. Protective effects of fish oil on carbon tetrachloride induced hepatotoxicity in rabbits. Int`l J Sci Basic Appl Res 2015; 19:400–408.
